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Image Search Results
Journal: Cell Host & Microbe
Article Title: Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors
doi: 10.1016/j.chom.2020.12.009
Figure Lengend Snippet:
Article Snippet: A total of 4.8 μg of ligated lentiGuidePurov2 Gordon et al. SARS-CoV-2 Human Interactome CRISPR Library plasmid DNA was electroporated into Endura electrocompetent cells (1.2 μg per electroporation) (Lucigen), recovered for 1 h at 37°C, plated across 20x 15-cm
Techniques: Virus, Recombinant, DNA Purification, Plasmid Preparation, Gel Extraction, CRISPR, Oligonucleotide Synthesis, Variant Assay, Modification, Software, Genome Wide, Knock-Out, Inverted Microscopy
Journal: PLoS ONE
Article Title: Characterization of the human homozygous R182W POLG2 mutation in mitochondrial DNA depletion syndrome
doi: 10.1371/journal.pone.0203198
Figure Lengend Snippet: (A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for POLG and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.
Article Snippet: 10 μL Real Time PCR reactions containing 5 μL 2X TaqMan Universal PCR master mix no AmpErase UNG (Applied Biosystems), 0.5 μL 20X TaqMan Primer–Probe set ( ACTB cat #: Hs1060665_g1, POLG cat #:
Techniques: Control, Real-time Polymerase Chain Reaction, Amplification