20x dna binding dye sample loading reagent Search Results


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Thermo Fisher 20x thermo sequenase dna polymerase
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Lb Carbenicillin Plates, supplied by Teknova, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher gene exp polg hs00160298 m1
(A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for <t>POLG</t> and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.
Gene Exp Polg Hs00160298 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher prehybridization buffer
(A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for <t>POLG</t> and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.
Prehybridization Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher nupagetm mops sds running buffer 20x invitrogen cat np0001
(A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for <t>POLG</t> and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.
Nupagetm Mops Sds Running Buffer 20x Invitrogen Cat Np0001, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluidigm 20x dna bingding dye sample loading reagent
(A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for <t>POLG</t> and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.
20x Dna Bingding Dye Sample Loading Reagent, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher recombinant proteins nuclei buffer 20x 10x genomics pn 2000153 digitonin thermo fisher scientific bn2006 trizma hydrochloride solution
(A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for <t>POLG</t> and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.
Recombinant Proteins Nuclei Buffer 20x 10x Genomics Pn 2000153 Digitonin Thermo Fisher Scientific Bn2006 Trizma Hydrochloride Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher tev buffer
(A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for <t>POLG</t> and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.
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Image Search Results


Journal: Cell Host & Microbe

Article Title: Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors

doi: 10.1016/j.chom.2020.12.009

Figure Lengend Snippet:

Article Snippet: A total of 4.8 μg of ligated lentiGuidePurov2 Gordon et al. SARS-CoV-2 Human Interactome CRISPR Library plasmid DNA was electroporated into Endura electrocompetent cells (1.2 μg per electroporation) (Lucigen), recovered for 1 h at 37°C, plated across 20x 15-cm LB-Carbenicillin plates (Teknova), and incubated at 37°C for 16 h. The total number of bacterial colonies per sub-pool was quantified using serial dilution plates to ensure a library representation of > 10,000-fold.

Techniques: Virus, Recombinant, DNA Purification, Plasmid Preparation, Gel Extraction, CRISPR, Oligonucleotide Synthesis, Variant Assay, Modification, Software, Genome Wide, Knock-Out, Inverted Microscopy

(A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for POLG and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.

Journal: PLoS ONE

Article Title: Characterization of the human homozygous R182W POLG2 mutation in mitochondrial DNA depletion syndrome

doi: 10.1371/journal.pone.0203198

Figure Lengend Snippet: (A) 8-day growth curves comparing control (black lines) and patient (red lines) dermal fibroblasts grown in glucose. Every point on the curves is the average of three independent growths. (B) MtDNA copy number was determined by quantitative PCR of the mitochondrial genes ND1 and ND4 and normalized to the nuclear gene RPPH1 , as described in Materials and methods. MtDNA copy number for control fibroblasts (black circles– ND1 /black triangles-ND4) or patient dermal fibroblasts (red circles- ND1 /red triangles- ND4 ) are shown. Standard curves of plasmids containing the amplified regions of interest allowed for absolute copy number to be determined by real time PCR. Experiments were done in triplicate, and N = 5 for each data point. (C) Transcript levels for POLG and POLG2 in patient fibroblasts were determined by quantitative PCR and normalized to ACTB as described in Materials and methods. WT p55 (black circles– POLG /black triangles– POLG2 ) R182W p55 (Red circles– POLG /red triangles– POLG2 ). Experiments were done in triplicate, and N = 5 for each data point.

Article Snippet: 10 μL Real Time PCR reactions containing 5 μL 2X TaqMan Universal PCR master mix no AmpErase UNG (Applied Biosystems), 0.5 μL 20X TaqMan Primer–Probe set ( ACTB cat #: Hs1060665_g1, POLG cat #: Hs00160298_m1, POLG2 cat #: Hs00945167_m1, Applied Biosystems), and 0.5 μL 1:2 diluted cDNA was setup in a 384-well plate.

Techniques: Control, Real-time Polymerase Chain Reaction, Amplification